Detailed information of TA system TA098763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3355123..3355754
• Replicon: chromosome
• Accession: NZ_CP027437
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: C6W65_RS17045
• Protein ID: WP_106906522.1
• Coordinates: 3355123..3355398 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: C6W65_RS17050
• Protein ID: WP_000593555.1
• Coordinates: 3355395..3355754 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS17030	3350127..3351194	+	1068	WP_106906520.1	HlyD family secretion protein	-
C6W65_RS17035	3351191..3353926	+	2736	WP_073461816.1	ribosome-associated ATPase/putative transporter RbbA	-
C6W65_RS17040	3353926..3355050	+	1125	WP_106906521.1	ABC transporter permease	-
C6W65_RS17045	3355123..3355398	+	276	WP_106906522.1	type II toxin-antitoxin system HicA family toxin	Toxin
C6W65_RS17050	3355395..3355754	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C6W65_RS17055	3355804..3356664	-	861	WP_001600635.1	class II aldolase	-
C6W65_RS17060	3356695..3356964	-	270	WP_000949583.1	HPr family phosphocarrier protein	-
C6W65_RS17065	3356954..3358462	-	1509	WP_106906523.1	carbohydrate kinase	-
C6W65_RS17070	3358455..3359813	-	1359	WP_106906524.1	PTS galactitol transporter subunit IIC	-
C6W65_RS17075	3359890..3360171	-	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
C6W65_RS17080	3360168..3360641	-	474	WP_001306344.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10165.79 Da
• Isoelectric Point: 10.6128

>T98763 WP_106906522.1 NZ_CP027437:3355123-3355398 [Escherichia coli]
MRTQVQALRKKQKNTLDQIVKSHVPQGIKWSDIESLVKALGGEIKEGRGSRCKFTLNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T98763 NZ_CP027437:3355123-3355398 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAATCTCATGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCACACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT98763 WP_000593555.1 NZ_CP027437:3355395-3355754 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT98763 NZ_CP027437:3355395-3355754 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6