Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 3217572..3218184 Replicon chromosome
Accession NZ_CP027437
Organism Escherichia coli strain 2012C-4221

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag C6W65_RS16385 Protein ID WP_000833473.1
Coordinates 3217572..3217757 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A5F1W1Z0
Locus tag C6W65_RS16390 Protein ID WP_000499743.1
Coordinates 3217774..3218184 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W65_RS16365 3213087..3214238 + 1152 WP_000741501.1 L-threonine dehydrogenase -
C6W65_RS16370 3214439..3215977 + 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
C6W65_RS16375 3216018..3217097 - 1080 WP_000061477.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
C6W65_RS16385 3217572..3217757 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
C6W65_RS16390 3217774..3218184 + 411 WP_000499743.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6W65_RS16395 3218256..3220220 - 1965 WP_096098550.1 glycoside hydrolase family 127 protein -
C6W65_RS16400 3220231..3221631 - 1401 WP_106906503.1 MFS transporter -
C6W65_RS16405 3221857..3222672 + 816 WP_000891823.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T98762 WP_000833473.1 NZ_CP027437:3217572-3217757 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T98762 NZ_CP027437:3217572-3217757 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15213.09 Da        Isoelectric Point: 4.5486

>AT98762 WP_000499743.1 NZ_CP027437:3217774-3218184 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGA

Download         Length: 411 bp

>AT98762 NZ_CP027437:3217774-3218184 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1W1Z0

References