Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2584563..2585060 Replicon chromosome
Accession NZ_CP027437
Organism Escherichia coli strain 2012C-4221

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6W65_RS13355 Protein ID WP_001173343.1
Coordinates 2584563..2584736 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W4RHQ7
Locus tag C6W65_RS13360 Protein ID WP_001600874.1
Coordinates 2584764..2585060 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W65_RS13345 2581233..2582690 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
C6W65_RS13350 2582927..2584444 + 1518 WP_000003806.1 lysine--tRNA ligase -
C6W65_RS13355 2584563..2584736 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6W65_RS13360 2584764..2585060 - 297 WP_001600874.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6W65_RS13365 2585289..2585561 - 273 WP_106906401.1 N-acetyltransferase -
C6W65_RS13370 2585573..2585803 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
C6W65_RS13375 2585984..2587615 + 1632 WP_106906402.1 sensor histidine kinase -
C6W65_RS13380 2587612..2588331 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98761 WP_001173343.1 NZ_CP027437:c2584736-2584563 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98761 NZ_CP027437:c2584736-2584563 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.00 Da        Isoelectric Point: 4.1705

>AT98761 WP_001600874.1 NZ_CP027437:c2585060-2584764 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRTLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98761 NZ_CP027437:c2585060-2584764 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGAACATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGATTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W4RHQ7

References