Detailed information of TA system TA098760

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2489171..2489766
• Replicon: chromosome
• Accession: NZ_CP027437
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: C6W65_RS12835
• Protein ID: WP_000239581.1
• Coordinates: 2489171..2489521 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: C6W65_RS12840
• Protein ID: WP_001223213.1
• Coordinates: 2489515..2489766 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS12815	2484625..2485647	-	1023	WP_001361374.1	ABC transporter permease	-
C6W65_RS12820	2485661..2487163	-	1503	WP_001095660.1	sugar ABC transporter ATP-binding protein	-
C6W65_RS12825	2487296..2488252	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
C6W65_RS12830	2488562..2489092	+	531	WP_000055075.1	inorganic diphosphatase	-
C6W65_RS12835	2489171..2489521	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
C6W65_RS12840	2489515..2489766	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
C6W65_RS12845	2489978..2490319	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
C6W65_RS12850	2490322..2494101	-	3780	WP_064226490.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T98760 WP_000239581.1 NZ_CP027437:c2489521-2489171 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T98760 NZ_CP027437:c2489521-2489171 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT98760 WP_001223213.1 NZ_CP027437:c2489766-2489515 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT98760 NZ_CP027437:c2489766-2489515 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAATAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7