Detailed information of TA system TA098758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2202005..2202522
• Replicon: chromosome
• Accession: NZ_CP027437
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: C6W65_RS11330
• Protein ID: WP_106906324.1
• Coordinates: 2202005..2202286 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1NMQ0
• Locus tag: C6W65_RS11335
• Protein ID: WP_000125568.1
• Coordinates: 2202289..2202522 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS11305	2197647..2198933	+	1287	WP_000800470.1	peptidylprolyl isomerase SurA	-
C6W65_RS11310	2198933..2199922	+	990	WP_000241267.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
C6W65_RS11315	2199919..2200740	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
C6W65_RS11320	2200743..2201120	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
C6W65_RS11325	2201127..2201975	+	849	WP_106906323.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
C6W65_RS11330	2202005..2202286	-	282	WP_106906324.1	CcdB family protein	Toxin
C6W65_RS11335	2202289..2202522	-	234	WP_000125568.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
C6W65_RS11340	2202608..2203087	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
C6W65_RS11345	2203279..2205141	-	1863	WP_000377115.1	glutathione-regulated potassium-efflux system protein KefC	-
C6W65_RS11350	2205134..2205664	-	531	WP_106906325.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
C6W65_RS11355	2205772..2207103	-	1332	WP_001183198.1	MFS transporter	-
C6W65_RS11360	2207162..2207449	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-93)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10550.34 Da
• Isoelectric Point: 9.9120

>T98758 WP_106906324.1 NZ_CP027437:c2202286-2202005 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLI

Nucleotide

• Length: 282 bp

>T98758 NZ_CP027437:c2202286-2202005 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.55 Da
• Isoelectric Point: 5.8062

>AT98758 WP_000125568.1 NZ_CP027437:c2202522-2202289 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT98758 NZ_CP027437:c2202522-2202289 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A829GEF2