Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 5164336..5164967 Replicon chromosome
Accession NZ_CP027435
Organism Escherichia coli strain 2014C-3599

Toxin (Protein)


Gene name hicA Uniprot ID A0A377MSE5
Locus tag ND02_RS27550 Protein ID WP_001259383.1
Coordinates 5164692..5164967 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ND02_RS27545 Protein ID WP_000593555.1
Coordinates 5164336..5164695 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND02_RS27520 5160468..5161412 + 945 WP_000947089.1 nickel ABC transporter permease subunit NikB -
ND02_RS27525 5161409..5162242 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
ND02_RS27530 5162242..5163006 + 765 WP_001136244.1 nickel import ATP-binding protein NikD -
ND02_RS27535 5163003..5163809 + 807 WP_000173673.1 nickel import ATP-binding protein NikE -
ND02_RS27540 5163815..5164216 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
ND02_RS27545 5164336..5164695 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ND02_RS27550 5164692..5164967 - 276 WP_001259383.1 type II toxin-antitoxin system HicA family toxin Toxin
ND02_RS27555 5165040..5166164 - 1125 WP_001343434.1 ABC transporter permease -
ND02_RS27560 5166164..5168899 - 2736 WP_000149120.1 ribosome-associated ATPase/putative transporter RbbA -
ND02_RS27565 5168896..5169963 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10209.98 Da        Isoelectric Point: 10.7076

>T98743 WP_001259383.1 NZ_CP027435:c5164967-5164692 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGPRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T98743 NZ_CP027435:c5164967-5164692 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTCCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT98743 WP_000593555.1 NZ_CP027435:c5164695-5164336 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT98743 NZ_CP027435:c5164695-5164336 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377MSE5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References