Detailed information of TA system TA098740

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4698865..4699703
• Replicon: chromosome
• Accession: NZ_CP027435
• Organism: Escherichia coli strain 2014C-3599

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: ND02_RS24935
• Protein ID: WP_106902990.1
• Coordinates: 4698865..4699245 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ND02_RS24940
• Protein ID: WP_001285487.1
• Coordinates: 4699335..4699703 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND02_RS24905	4694507..4696348	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
ND02_RS24910	4696427..4696933	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
ND02_RS24920	4697233..4698078	-	846	WP_001274557.1	DUF4942 domain-containing protein	-
ND02_RS24925	4698163..4698360	-	198	WP_000772026.1	DUF957 domain-containing protein	-
ND02_RS24930	4698380..4698868	-	489	WP_001054228.1	hypothetical protein	-
ND02_RS24935	4698865..4699245	-	381	WP_106902990.1	TA system toxin CbtA family protein	Toxin
ND02_RS24940	4699335..4699703	-	369	WP_001285487.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ND02_RS24945	4699779..4700000	-	222	WP_000692312.1	DUF987 domain-containing protein	-
ND02_RS24950	4700063..4700539	-	477	WP_001186728.1	RadC family protein	-
ND02_RS24955	4700555..4701028	-	474	WP_000855068.1	antirestriction protein	-
ND02_RS24960	4701122..4701367	-	246	WP_001164974.1	hypothetical protein	-
ND02_RS24965	4701367..4701807	-	441	Protein_4578	DUF932 domain-containing protein	-
ND02_RS24970	4701926..4703704	-	1779	WP_001243192.1	reverse transcriptase/maturase family protein	-
ND02_RS24975	4704214..4704597	-	384	Protein_4580	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4686153..4717649	31496

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14303.26 Da
• Isoelectric Point: 6.9578

>T98740 WP_106902990.1 NZ_CP027435:c4699245-4698865 [Escherichia coli]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T98740 NZ_CP027435:c4699245-4698865 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.53 Da
• Isoelectric Point: 5.4844

>AT98740 WP_001285487.1 NZ_CP027435:c4699703-4699335 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT98740 NZ_CP027435:c4699703-4699335 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA