Detailed information of TA system TA098739

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4575395..4576179
• Replicon: chromosome
• Accession: NZ_CP027435
• Organism: Escherichia coli strain 2014C-3599

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: C7BUQ8
• Locus tag: ND02_RS24265
• Protein ID: WP_000854717.1
• Coordinates: 4575853..4576179 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ND02_RS24260
• Protein ID: WP_001285487.1
• Coordinates: 4575395..4575763 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND02_RS24220	4571271..4571444	-	174	Protein_4443	N-acetyltransferase	-
ND02_RS24225	4571935..4572141	+	207	WP_000632607.1	AlpA family transcriptional regulator	-
ND02_RS24230	4572240..4573417	+	1178	WP_085968792.1	IS3 family transposase	-
ND02_RS24235	4573423..4573731	+	309	Protein_4446	DUF932 domain-containing protein	-
ND02_RS24240	4573731..4573976	+	246	WP_032212706.1	hypothetical protein	-
ND02_RS24245	4574070..4574543	+	474	WP_000855068.1	antirestriction protein	-
ND02_RS24250	4574559..4575035	+	477	WP_001186728.1	RadC family protein	-
ND02_RS24255	4575098..4575319	+	222	WP_000692312.1	DUF987 domain-containing protein	-
ND02_RS24260	4575395..4575763	+	369	WP_001285487.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ND02_RS24265	4575853..4576179	+	327	WP_000854717.1	TA system toxin CbtA family protein	Toxin
ND02_RS24270	4576227..4576715	+	489	WP_000761730.1	hypothetical protein	-
ND02_RS24275	4576727..4576924	+	198	WP_000839243.1	DUF957 domain-containing protein	-
ND02_RS24280	4577009..4577851	+	843	WP_024174205.1	DUF4942 domain-containing protein	-
ND02_RS24285	4578194..4578730	-	537	WP_000942788.1	GspM family type II secretion system protein YghD	-
ND02_RS24290	4578732..4579910	-	1179	WP_000094965.1	type II secretion system protein GspL	-
ND02_RS24295	4579907..4580884	-	978	WP_000633206.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12194.91 Da
• Isoelectric Point: 6.4697

>T98739 WP_000854717.1 NZ_CP027435:4575853-4576179 [Escherichia coli]
MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDN

Nucleotide

• Length: 327 bp

>T98739 NZ_CP027435:4575853-4576179 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13737.53 Da
• Isoelectric Point: 5.4844

>AT98739 WP_001285487.1 NZ_CP027435:4575395-4575763 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT98739 NZ_CP027435:4575395-4575763 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: C7BUQ8