Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4524355..4525153 Replicon chromosome
Accession NZ_CP027435
Organism Escherichia coli strain 2014C-3599

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3W5Y7S6
Locus tag ND02_RS23945 Protein ID WP_000854916.1
Coordinates 4524776..4525153 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3W5Y7S1
Locus tag ND02_RS23940 Protein ID WP_024174207.1
Coordinates 4524355..4524729 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND02_RS23900 4519933..4520175 - 243 WP_096855415.1 transposase -
ND02_RS29590 4520417..4520686 + 270 Protein_4380 transposase -
ND02_RS29595 4520691..4521157 - 467 Protein_4381 transposase -
ND02_RS23910 4521195..4521548 + 354 Protein_4382 transposase -
ND02_RS23915 4521680..4522105 + 426 WP_000422707.1 IS66 family insertion sequence hypothetical protein -
ND02_RS23920 4522102..4522452 + 351 WP_000624681.1 IS66 family insertion sequence element accessory protein TnpB -
ND02_RS23925 4522530..4523727 - 1198 Protein_4385 IS3 family transposase -
ND02_RS23930 4523768..4523902 + 135 Protein_4386 hypothetical protein -
ND02_RS23935 4523971..4524192 + 222 WP_000692312.1 DUF987 domain-containing protein -
ND02_RS23940 4524355..4524729 + 375 WP_024174207.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ND02_RS23945 4524776..4525153 + 378 WP_000854916.1 TA system toxin CbtA family protein Toxin
ND02_RS23950 4525150..4525638 + 489 WP_000777666.1 hypothetical protein -
ND02_RS23955 4525650..4525847 + 198 WP_000839246.1 DUF957 domain-containing protein -
ND02_RS29775 4525932..4526090 + 159 WP_000014512.1 hypothetical protein -
ND02_RS23960 4526108..4527664 - 1557 WP_001066419.1 IS66 family transposase -
ND02_RS23965 4527684..4528031 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
ND02_RS23970 4528028..4528702 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
ND02_RS23975 4528778..4529491 + 714 Protein_4396 DUF4942 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae 4513195..4545784 32589
- inside IScluster/Tn - - 4515663..4530993 15330


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14033.04 Da        Isoelectric Point: 9.1378

>T98738 WP_000854916.1 NZ_CP027435:4524776-4525153 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ

Download         Length: 378 bp

>T98738 NZ_CP027435:4524776-4525153 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13789.59 Da        Isoelectric Point: 6.7387

>AT98738 WP_024174207.1 NZ_CP027435:4524355-4524729 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS

Download         Length: 375 bp

>AT98738 NZ_CP027435:4524355-4524729 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5Y7S6

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y7S1

References