Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4436032..4436692 Replicon chromosome
Accession NZ_CP027435
Organism Escherichia coli strain 2014C-3599

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag ND02_RS23435 Protein ID WP_000244778.1
Coordinates 4436032..4436445 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ND02_RS23440 Protein ID WP_000354046.1
Coordinates 4436426..4436692 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND02_RS23415 4431996..4433729 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
ND02_RS23420 4433735..4434445 - 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ND02_RS23425 4434469..4435365 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ND02_RS23430 4435477..4435998 + 522 WP_001055874.1 flavodoxin FldB -
ND02_RS23435 4436032..4436445 - 414 WP_000244778.1 protein YgfX Toxin
ND02_RS23440 4436426..4436692 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ND02_RS23445 4436935..4437915 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ND02_RS23450 4437992..4438651 - 660 WP_000250274.1 hemolysin III family protein -
ND02_RS23455 4438815..4439126 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
ND02_RS23460 4439171..4440604 + 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
ND02_RS23465 4440661..4441404 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T98737 WP_000244778.1 NZ_CP027435:c4436445-4436032 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

>T98737 NZ_CP027435:c4436445-4436032 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98737 WP_000354046.1 NZ_CP027435:c4436692-4436426 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98737 NZ_CP027435:c4436692-4436426 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References