Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2192145..2192616 Replicon chromosome
Accession NZ_CP027435
Organism Escherichia coli strain 2014C-3599

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ND02_RS11370 Protein ID WP_001303511.1
Coordinates 2192145..2192423 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ND02_RS11375 Protein ID WP_001302048.1
Coordinates 2192425..2192616 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND02_RS11345 2189845..2190435 - 591 Protein_2048 exonuclease -
ND02_RS11350 2190559..2190780 - 222 WP_000560212.1 cell division protein FtsZ -
ND02_RS11360 2191493..2191792 - 300 WP_001240334.1 hypothetical protein -
ND02_RS11370 2192145..2192423 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ND02_RS11375 2192425..2192616 - 192 WP_001302048.1 hypothetical protein Antitoxin
ND02_RS11380 2192637..2193008 - 372 WP_001169687.1 hypothetical protein -
ND02_RS11385 2193106..2193408 + 303 WP_000172738.1 transcriptional regulator -
ND02_RS11390 2193405..2193830 + 426 WP_000693944.1 Rha family transcriptional regulator -
ND02_RS11395 2193853..2194815 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
ND02_RS11400 2194856..2195278 + 423 WP_001151187.1 DUF977 family protein -
ND02_RS11405 2195384..2195596 + 213 WP_000935423.1 hypothetical protein -
ND02_RS11410 2195629..2195847 + 219 WP_000209148.1 DUF4014 family protein -
ND02_RS29305 2195849..2196007 + 159 WP_001370098.1 hypothetical protein -
ND02_RS11415 2196068..2197567 - 1500 WP_106902979.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2181431..2231694 50263


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T98719 WP_001303511.1 NZ_CP027435:c2192423-2192145 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98719 NZ_CP027435:c2192423-2192145 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98719 WP_001302048.1 NZ_CP027435:c2192616-2192425 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98719 NZ_CP027435:c2192616-2192425 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References