Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 559135..559632 Replicon chromosome
Accession NZ_CP027435
Organism Escherichia coli strain 2014C-3599

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ND02_RS02915 Protein ID WP_001173343.1
Coordinates 559459..559632 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A3W5Y3H9
Locus tag ND02_RS02910 Protein ID WP_071532590.1
Coordinates 559135..559431 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND02_RS02885 555866..556585 - 720 WP_000611288.1 two-component system response regulator DcuR -
ND02_RS02890 556582..558213 - 1632 WP_001216468.1 sensor histidine kinase -
ND02_RS02895 558394..558624 + 231 WP_000371698.1 (4Fe-4S)-binding protein -
ND02_RS02900 558636..558908 + 273 WP_000405647.1 N-acetyltransferase -
ND02_RS02910 559135..559431 + 297 WP_071532590.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ND02_RS02915 559459..559632 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ND02_RS02920 559751..561268 - 1518 WP_001295082.1 lysine--tRNA ligase -
ND02_RS02925 561505..562962 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98714 WP_001173343.1 NZ_CP027435:559459-559632 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98714 NZ_CP027435:559459-559632 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11482.04 Da        Isoelectric Point: 4.2931

>AT98714 WP_071532590.1 NZ_CP027435:559135-559431 [Escherichia coli]
MEGKKKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98714 NZ_CP027435:559135-559431 [Escherichia coli]
ATGGAAGGTAAAAAAAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y3H9

References