Detailed information of TA system TA098679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2778988..2779607
• Replicon: chromosome
• Accession: NZ_CP027426
• Organism: Klebsiella oxytoca strain FDAARGOS_335

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: CEQ13_RS14645
• Protein ID: WP_004099646.1
• Coordinates: 2778988..2779206 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: CEQ13_RS14650
• Protein ID: WP_004099648.1
• Coordinates: 2779233..2779607 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ13_RS14615	2775025..2775288	+	264	WP_004099638.1	type B 50S ribosomal protein L31	-
CEQ13_RS14620	2775288..2775428	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
CEQ13_RS14625	2775425..2776123	-	699	WP_105889820.1	GNAT family N-acetyltransferase	-
CEQ13_RS14630	2776225..2777679	+	1455	WP_105889821.1	PLP-dependent aminotransferase family protein	-
CEQ13_RS14635	2777654..2778124	-	471	WP_004099643.1	YlaC family protein	-
CEQ13_RS14640	2778261..2778827	-	567	WP_004099644.1	maltose O-acetyltransferase	-
CEQ13_RS14645	2778988..2779206	-	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
CEQ13_RS14650	2779233..2779607	-	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
CEQ13_RS14655	2780089..2783235	-	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
CEQ13_RS14660	2783258..2784451	-	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T98679 WP_004099646.1 NZ_CP027426:c2779206-2778988 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T98679 NZ_CP027426:c2779206-2778988 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT98679 WP_004099648.1 NZ_CP027426:c2779607-2779233 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT98679 NZ_CP027426:c2779607-2779233 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3H713