Detailed information of TA system TA098674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 126702..127438
• Replicon: plasmid unnamed
• Accession: NZ_CP027425
• Organism: Klebsiella oxytoca strain FDAARGOS_335

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: CEQ13_RS00755
• Protein ID: WP_003026803.1
• Coordinates: 126956..127438 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: CEQ13_RS00750
• Protein ID: WP_003026799.1
• Coordinates: 126702..126968 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ13_RS00705	122764..123126	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
CEQ13_RS00710	123176..123526	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
CEQ13_RS00715	123884..124153	+	270	WP_004152102.1	hypothetical protein	-
CEQ13_RS00720	124141..124716	+	576	WP_004152103.1	hypothetical protein	-
CEQ13_RS00725	124747..125241	+	495	WP_004152104.1	DNA-binding protein	-
CEQ13_RS00730	125285..125653	+	369	WP_004152105.1	hypothetical protein	-
CEQ13_RS00735	125687..125890	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
CEQ13_RS00740	125939..126196	+	258	WP_004152107.1	hypothetical protein	-
CEQ13_RS00745	126272..126526	+	255	WP_004152108.1	hypothetical protein	-
CEQ13_RS00750	126702..126968	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
CEQ13_RS00755	126956..127438	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
CEQ13_RS00760	127597..128851	-	1255	Protein_135	IS3 family transposase	-
CEQ13_RS00765	128939..129901	-	963	WP_004152113.1	M48 family metalloprotease	-
CEQ13_RS00770	129888..130637	-	750	WP_009483782.1	phosphate-starvation-inducible PsiE family protein	-
CEQ13_RS00780	130875..131072	-	198	WP_004152115.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..195881	195881

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T98674 WP_003026803.1 NZ_CP027425:126956-127438 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T98674 NZ_CP027425:126956-127438 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT98674 WP_003026799.1 NZ_CP027425:126702-126968 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT98674 NZ_CP027425:126702-126968 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66