Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 126702..127438 Replicon plasmid unnamed
Accession NZ_CP027425
Organism Klebsiella oxytoca strain FDAARGOS_335

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CEQ13_RS00755 Protein ID WP_003026803.1
Coordinates 126956..127438 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CEQ13_RS00750 Protein ID WP_003026799.1
Coordinates 126702..126968 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ13_RS00705 122764..123126 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
CEQ13_RS00710 123176..123526 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CEQ13_RS00715 123884..124153 + 270 WP_004152102.1 hypothetical protein -
CEQ13_RS00720 124141..124716 + 576 WP_004152103.1 hypothetical protein -
CEQ13_RS00725 124747..125241 + 495 WP_004152104.1 DNA-binding protein -
CEQ13_RS00730 125285..125653 + 369 WP_004152105.1 hypothetical protein -
CEQ13_RS00735 125687..125890 + 204 WP_004152106.1 hemolysin expression modulator Hha -
CEQ13_RS00740 125939..126196 + 258 WP_004152107.1 hypothetical protein -
CEQ13_RS00745 126272..126526 + 255 WP_004152108.1 hypothetical protein -
CEQ13_RS00750 126702..126968 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CEQ13_RS00755 126956..127438 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CEQ13_RS00760 127597..128851 - 1255 Protein_135 IS3 family transposase -
CEQ13_RS00765 128939..129901 - 963 WP_004152113.1 M48 family metalloprotease -
CEQ13_RS00770 129888..130637 - 750 WP_009483782.1 phosphate-starvation-inducible PsiE family protein -
CEQ13_RS00780 130875..131072 - 198 WP_004152115.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..195881 195881


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T98674 WP_003026803.1 NZ_CP027425:126956-127438 [Klebsiella oxytoca]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T98674 NZ_CP027425:126956-127438 [Klebsiella oxytoca]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT98674 WP_003026799.1 NZ_CP027425:126702-126968 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT98674 NZ_CP027425:126702-126968 [Klebsiella oxytoca]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References