Detailed information of TA system TA098654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagD-vapB/VapC-VagC
• Location: 38751..39400
• Replicon: plasmid unnamed1
• Accession: NZ_CP027406
• Organism: Roseobacter denitrificans strain FDAARGOS_309

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CEP88_RS00150
• Protein ID: WP_011655384.1
• Coordinates: 38751..39173 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q07GS2
• Locus tag: CEP88_RS00155
• Protein ID: WP_011655383.1
• Coordinates: 39170..39400 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEP88_RS00120	34067..34447	-	381	WP_044033566.1	DUF5615 family PIN-like protein	-
CEP88_RS00125	34457..35056	-	600	WP_050759150.1	DUF433 domain-containing protein	-
CEP88_RS00130	35259..35888	-	630	WP_011655389.1	SMC-Scp complex subunit ScpB	-
CEP88_RS00135	35885..36847	-	963	WP_011655388.1	DUF1403 family protein	-
CEP88_RS00140	36972..38186	+	1215	WP_011655387.1	tyrosine-type recombinase/integrase	-
CEP88_RS00145	38240..38545	+	306	WP_011655385.1	hypothetical protein	-
CEP88_RS00150	38751..39173	-	423	WP_011655384.1	type II toxin-antitoxin system VapC family toxin	Toxin
CEP88_RS00155	39170..39400	-	231	WP_011655383.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CEP88_RS00160	39476..40789	-	1314	WP_011655382.1	replication protein C	-
CEP88_RS00165	41030..42052	-	1023	WP_011655381.1	ParB N-terminal domain-containing protein	-
CEP88_RS00170	42049..43404	-	1356	WP_011655380.1	AAA family ATPase	-
CEP88_RS00175	43602..44168	-	567	WP_044033563.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106464	106464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15527.57 Da
• Isoelectric Point: 4.4225

>T98654 WP_011655384.1 NZ_CP027406:c39173-38751 [Roseobacter denitrificans]
MTLYMLDTDICSYLMREHPAAVLRAMEKQVEAGEDICISVITYSELRLGAARSQARAKYDGLIDALSDRLDFIADWSTKE
ADRFADVQSHLLGQGAPIGANDTMIAAHALSLGAVLVTNNQRHFSKIEELVLENWLDQSD

Nucleotide

• Length: 423 bp

>T98654 NZ_CP027406:c39173-38751 [Roseobacter denitrificans]
TTGACGCTCTACATGCTCGATACGGACATTTGCAGCTATCTGATGCGTGAGCATCCGGCGGCGGTGTTACGAGCAATGGA
AAAGCAGGTCGAGGCGGGGGAGGATATCTGCATTTCCGTGATTACTTACTCGGAACTGCGCCTCGGGGCGGCCCGGTCTC
AGGCGCGTGCTAAATATGACGGTTTGATCGATGCGCTGTCTGACCGGCTCGACTTCATCGCGGATTGGTCGACGAAAGAA
GCTGATCGGTTTGCAGACGTTCAGTCCCATTTGCTGGGGCAAGGGGCCCCGATTGGTGCCAATGACACGATGATCGCCGC
CCATGCTCTGTCGCTGGGGGCAGTCTTGGTCACGAATAACCAACGTCATTTTTCTAAGATCGAAGAACTGGTGCTGGAAA
ACTGGCTGGACCAATCTGATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8554.88 Da
• Isoelectric Point: 8.7696

>AT98654 WP_011655383.1 NZ_CP027406:c39400-39170 [Roseobacter denitrificans]
MTAIAKLFTNGRSQAVRIPKDFAFHGIDEVSVRKIGNKLVLEPIRKTWTSLADEEPAAADFMAERPDLLNPDRVKF

Nucleotide

• Length: 231 bp

>AT98654 NZ_CP027406:c39400-39170 [Roseobacter denitrificans]
ATGACCGCAATCGCAAAACTGTTCACCAATGGACGCAGCCAAGCCGTGCGGATTCCGAAGGATTTCGCCTTTCACGGTAT
CGATGAGGTTTCGGTCCGTAAGATCGGGAACAAGCTGGTGTTGGAACCGATCCGCAAGACATGGACCAGCCTGGCCGACG
AAGAGCCCGCGGCGGCGGATTTCATGGCGGAGCGTCCCGACTTGCTGAACCCAGACAGAGTCAAGTTTTGA

Structures

Antitoxin

• AlphaFold DB: Q07GS2