Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1498351..1498922 Replicon chromosome
Accession NZ_CP027402
Organism Enterococcus faecium strain FDAARGOS_323

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag CEQ01_RS08485 Protein ID WP_002286801.1
Coordinates 1498351..1498692 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag CEQ01_RS08490 Protein ID WP_002323011.1
Coordinates 1498692..1498922 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ01_RS08455 1493561..1494895 - 1335 WP_002286788.1 ABC transporter substrate-binding protein -
CEQ01_RS08460 1495103..1495933 + 831 WP_002286789.1 manganese catalase family protein -
CEQ01_RS08470 1496177..1496459 - 283 Protein_1417 transposase -
CEQ01_RS08475 1496617..1496811 - 195 WP_002295146.1 hypothetical protein -
CEQ01_RS08480 1497316..1497501 - 186 WP_002304207.1 hypothetical protein -
CEQ01_RS08485 1498351..1498692 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CEQ01_RS08490 1498692..1498922 - 231 WP_002323011.1 hypothetical protein Antitoxin
CEQ01_RS08500 1499209..1499547 + 339 WP_002286804.1 hypothetical protein -
CEQ01_RS08505 1499752..1500327 - 576 WP_002293673.1 SOS response-associated peptidase family protein -
CEQ01_RS08525 1500814..1501395 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
CEQ01_RS08530 1501578..1502204 - 627 WP_002286816.1 cysteine hydrolase -
CEQ01_RS08535 1502226..1503557 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T98650 WP_002286801.1 NZ_CP027402:c1498692-1498351 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T98650 NZ_CP027402:c1498692-1498351 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT98650 WP_002323011.1 NZ_CP027402:c1498922-1498692 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT98650 NZ_CP027402:c1498922-1498692 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References