Detailed information of TA system TA098649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1323048..1323619
• Replicon: chromosome
• Accession: NZ_CP027402
• Organism: Enterococcus faecium strain FDAARGOS_323

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CEQ01_RS07650
• Protein ID: WP_002322675.1
• Coordinates: 1323278..1323619 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CEQ01_RS07645
• Protein ID: WP_002322676.1
• Coordinates: 1323048..1323278 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ01_RS07630	1320010..1320822	-	813	WP_002322679.1	AAA family ATPase	-
CEQ01_RS07635	1320815..1322251	-	1437	WP_002322678.1	DDE-type integrase/transposase/recombinase	-
CEQ01_RS07640	1322223..1322816	-	594	WP_002322677.1	recombinase family protein	-
CEQ01_RS07645	1323048..1323278	+	231	WP_002322676.1	hypothetical protein	Antitoxin
CEQ01_RS07650	1323278..1323619	+	342	WP_002322675.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CEQ01_RS07655	1323833..1325335	-	1503	WP_002322674.1	xylulokinase	-
CEQ01_RS07660	1325408..1326460	-	1053	WP_002323515.1	sugar ABC transporter substrate-binding protein	-
CEQ01_RS07665	1326513..1327481	-	969	WP_002322672.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13232.43 Da
• Isoelectric Point: 9.4128

>T98649 WP_002322675.1 NZ_CP027402:1323278-1323619 [Enterococcus faecium]
MTEEKNYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIRNFPTRYSLPTELDTTGQILISQ
LKSLDFNERKLKKIESLPLQDMAKIDQMIEYIF

Nucleotide

• Length: 342 bp

>T98649 NZ_CP027402:1323278-1323619 [Enterococcus faecium]
ATGACTGAAGAAAAAAATTACATTCCTAAAAAGGGAGATATTGTTTGGATTGATTTTGATCCTTCTGCAGGAAAAGAGAT
TCAAAAGCGGCGTCCTGGATTAGTTGTTTCTAGATACGAGTTTAATCGCAAAACAATGTTTGCAGTTATTTGTCCGATTA
CCTCTACTATAAGAAATTTTCCGACACGCTATTCATTACCTACCGAATTAGACACTACTGGACAAATACTTATTTCTCAA
TTGAAGTCGCTCGATTTCAATGAGAGAAAACTTAAAAAAATTGAATCCTTACCTTTACAAGATATGGCGAAAATCGATCA
AATGATTGAATATATATTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8551.48 Da
• Isoelectric Point: 4.0070

>AT98649 WP_002322676.1 NZ_CP027402:1323048-1323278 [Enterococcus faecium]
MLITKSRLQGSSVVITLPSDNGKKPSENKEYIVIYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLSPEGREIL

Nucleotide

• Length: 231 bp

>AT98649 NZ_CP027402:1323048-1323278 [Enterococcus faecium]
ATGCTTATTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGATTACTTTGCCTTCTGATAACGGAAAGAAGCCTTCGGA
AAATAAAGAATATATAGTTATCTATTCTGATGATGGAACAATTACTTTGGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GAGAAGAAGCGGAGTATTACGAAAAAGATGAATGGGAAGATTTGTCTCCTGAAGGAAGAGAGATTTTATAA