Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 451461..452112 Replicon chromosome
Accession NZ_CP027398
Organism Providencia stuartii strain FDAARGOS_291

Toxin (Protein)


Gene name Hha Uniprot ID K8W3H1
Locus tag CEP70_RS01920 Protein ID WP_004927064.1
Coordinates 451461..451664 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID B2Q721
Locus tag CEP70_RS01925 Protein ID WP_004927067.1
Coordinates 451744..452112 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEP70_RS01895 447339..447677 + 339 WP_004927053.1 P-II family nitrogen regulator -
CEP70_RS01900 447688..448971 + 1284 WP_014656764.1 ammonium transporter AmtB -
CEP70_RS01905 449208..450074 - 867 WP_105874159.1 acyl-CoA thioesterase II -
CEP70_RS01910 450397..450855 + 459 WP_004927062.1 YbaY family lipoprotein -
CEP70_RS01920 451461..451664 - 204 WP_004927064.1 hemolysin expression modulator Hha Toxin
CEP70_RS01925 451744..452112 - 369 WP_004927067.1 Hha toxicity modulator TomB Antitoxin
CEP70_RS01930 452680..454014 - 1335 WP_072502514.1 murein transglycosylase D -
CEP70_RS01935 454093..454848 - 756 WP_004927081.1 hydroxyacylglutathione hydrolase -
CEP70_RS01940 454882..455607 + 726 WP_053110025.1 methyltransferase domain-containing protein -
CEP70_RS01945 455686..456156 - 471 WP_014656762.1 ribonuclease HI -
CEP70_RS01950 456211..456972 + 762 WP_004927092.1 DNA polymerase III subunit epsilon -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8075.37 Da        Isoelectric Point: 6.9770

>T98647 WP_004927064.1 NZ_CP027398:c451664-451461 [Providencia stuartii]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSEDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Download         Length: 204 bp

>T98647 NZ_CP027398:c451664-451461 [Providencia stuartii]
ATGACCAAAACTGATTATCTGATGCGTTTAAGAAAATGCACAACCATCGATACACTTGAGCGTGTTATTGAGAAAAATAA
GTATGAGCTTTCTGAAGATGAGTTAGAATTGTTCTATTCAGCTGCTGACCATCGCCTTGCAGAACTCACTATGAATAAAC
TTTATGACAAAATCCCTGCATCGGTTTGGAAATTTGTTAGGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14149.01 Da        Isoelectric Point: 4.2591

>AT98647 WP_004927067.1 NZ_CP027398:c452112-451744 [Providencia stuartii]
MDEYSPKNYDISELKYLCNSLNREAMLSLQKTNTHWVNDLSSPQSARLNELIEHIAAFVWQFKIKYPKENLVISLVEEYL
DETYDLFGSPVITLSEIIDWQSMNQNLVSVLDDDLKCPASKT

Download         Length: 369 bp

>AT98647 NZ_CP027398:c452112-451744 [Providencia stuartii]
ATGGATGAATATTCACCCAAAAATTATGATATATCTGAGTTAAAATACTTGTGTAACAGTTTGAATCGAGAAGCAATGTT
AAGCTTACAGAAAACTAATACACACTGGGTCAATGATTTAAGTTCACCACAAAGTGCTAGACTTAATGAACTCATTGAAC
ATATTGCAGCCTTCGTGTGGCAATTTAAGATAAAATATCCCAAAGAGAATCTCGTTATATCATTAGTTGAAGAGTATCTT
GATGAGACTTATGATTTATTCGGAAGCCCTGTAATAACTCTAAGTGAAATTATTGACTGGCAGAGCATGAATCAAAATCT
AGTTTCCGTACTTGATGATGATTTAAAGTGCCCTGCAAGCAAAACATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8W3H1

Antitoxin

Source ID Structure
AlphaFold DB A0A140NKN2

References