Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5171092..5171910 Replicon chromosome
Accession NZ_CP027394
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0E0Y102
Locus tag CEQ27_RS27835 Protein ID WP_000771609.1
Coordinates 5171536..5171910 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0E0Y470
Locus tag CEQ27_RS27830 Protein ID WP_001285568.1
Coordinates 5171092..5171445 (+) Length 118 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS27790 5167277..5167960 + 684 WP_000775510.1 hypothetical protein -
CEQ27_RS27795 5167976..5168386 + 411 WP_000846716.1 hypothetical protein -
CEQ27_RS27805 5168607..5169428 + 822 WP_001241709.1 DUF945 domain-containing protein -
CEQ27_RS27810 5169428..5169673 + 246 WP_001164966.1 hypothetical protein -
CEQ27_RS27815 5169767..5170240 + 474 WP_000855049.1 antirestriction protein -
CEQ27_RS27820 5170256..5170732 + 477 WP_001186756.1 RadC family protein -
CEQ27_RS27825 5170795..5171016 + 222 WP_000692357.1 DUF987 domain-containing protein -
CEQ27_RS27830 5171092..5171445 + 354 WP_001285568.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
CEQ27_RS27835 5171536..5171910 + 375 WP_000771609.1 TA system toxin CbtA family protein Toxin
CEQ27_RS27840 5171907..5172398 + 492 WP_000976860.1 hypothetical protein -
CEQ27_RS27845 5172410..5172607 + 198 WP_000839270.1 DUF957 domain-containing protein -
CEQ27_RS27850 5172692..5173534 + 843 WP_001274560.1 DUF4942 domain-containing protein -
CEQ27_RS27860 5174005..5174943 + 939 WP_000351313.1 glyoxylate/hydroxypyruvate reductase GhrA -
CEQ27_RS27865 5174998..5175735 + 738 WP_000283664.1 zinc-binding phosphatase -
CEQ27_RS27870 5175759..5176313 + 555 WP_001001902.1 molecular chaperone YcdY -
CEQ27_RS27875 5176415..5176906 + 492 WP_001297187.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(13-112)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14006.09 Da        Isoelectric Point: 7.2652

>T98624 WP_000771609.1 NZ_CP027394:5171536-5171910 [Escherichia coli O104:H4]
MKLLPDTHVREASCCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Download         Length: 375 bp

>T98624 NZ_CP027394:5171536-5171910 [Escherichia coli O104:H4]
ATGAAATTATTACCCGACACACATGTACGGGAGGCATCATGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 118 a.a.        Molecular weight: 13064.82 Da        Isoelectric Point: 5.9541

>AT98624 WP_001285568.1 NZ_CP027394:5171092-5171445 [Escherichia coli O104:H4]
VSDTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPR
HQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Download         Length: 354 bp

>AT98624 NZ_CP027394:5171092-5171445 [Escherichia coli O104:H4]
GTGTCAGACACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGCGC
CCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATG
CGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCCCGC
CATCAGCATACCGTCACGCTATATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCT
GGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y102

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y470

References