Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4377805..4378423 Replicon chromosome
Accession NZ_CP027394
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CEQ27_RS23665 Protein ID WP_001291435.1
Coordinates 4377805..4378023 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CEQ27_RS23670 Protein ID WP_000344800.1
Coordinates 4378049..4378423 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS23630 4373095..4373667 + 573 WP_000779826.1 lipoprotein -
CEQ27_RS23635 4373698..4374009 - 312 WP_000409911.1 MGMT family protein -
CEQ27_RS23645 4374388..4374741 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CEQ27_RS23650 4374783..4376333 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CEQ27_RS23655 4376497..4376967 - 471 WP_000136192.1 YlaC family protein -
CEQ27_RS23660 4377083..4377634 - 552 WP_000102564.1 maltose O-acetyltransferase -
CEQ27_RS23665 4377805..4378023 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CEQ27_RS23670 4378049..4378423 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CEQ27_RS23675 4378969..4382118 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CEQ27_RS23680 4382141..4383334 - 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98623 WP_001291435.1 NZ_CP027394:c4378023-4377805 [Escherichia coli O104:H4]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98623 NZ_CP027394:c4378023-4377805 [Escherichia coli O104:H4]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98623 WP_000344800.1 NZ_CP027394:c4378423-4378049 [Escherichia coli O104:H4]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98623 NZ_CP027394:c4378423-4378049 [Escherichia coli O104:H4]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References