Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3530407..3531208 Replicon chromosome
Accession NZ_CP027394
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag CEQ27_RS19265 Protein ID WP_001094430.1
Coordinates 3530407..3530784 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag CEQ27_RS19270 Protein ID WP_001285620.1
Coordinates 3530831..3531208 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS19240 3526491..3528029 - 1539 WP_001187182.1 lysine decarboxylation/transport transcriptional activator CadC -
CEQ27_RS19250 3528778..3529620 - 843 WP_001290187.1 DUF4942 domain-containing protein -
CEQ27_RS19255 3529705..3529902 - 198 WP_000772029.1 DUF957 domain-containing protein -
CEQ27_RS19260 3529922..3530410 - 489 WP_000761715.1 hypothetical protein -
CEQ27_RS19265 3530407..3530784 - 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
CEQ27_RS19270 3530831..3531208 - 378 WP_001285620.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
CEQ27_RS19275 3531288..3531509 - 222 WP_000692350.1 DUF987 domain-containing protein -
CEQ27_RS19280 3531596..3532071 - 476 Protein_3369 RadC family protein -
CEQ27_RS19285 3532087..3532560 - 474 WP_000855057.1 antirestriction protein -
CEQ27_RS29990 3532902..3533027 - 126 Protein_3371 DUF945 domain-containing protein -
CEQ27_RS19300 3533084..3533823 + 740 Protein_3372 IS3 family transposase -
CEQ27_RS19310 3534496..3536004 + 1509 WP_001189118.1 group II intron reverse transcriptase/maturase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T98619 WP_001094430.1 NZ_CP027394:c3530784-3530407 [Escherichia coli O104:H4]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T98619 NZ_CP027394:c3530784-3530407 [Escherichia coli O104:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT98619 WP_001285620.1 NZ_CP027394:c3531208-3530831 [Escherichia coli O104:H4]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT98619 NZ_CP027394:c3531208-3530831 [Escherichia coli O104:H4]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References