Detailed information of TA system TA098616

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2990558..2991359
• Replicon: chromosome
• Accession: NZ_CP027394
• Organism: Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0E0XS49
• Locus tag: CEQ27_RS16565
• Protein ID: WP_001094437.1
• Coordinates: 2990982..2991359 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0E0XTQ1
• Locus tag: CEQ27_RS16560
• Protein ID: WP_001390338.1
• Coordinates: 2990558..2990935 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ27_RS16525	2987676..2987834	+	159	WP_001323397.1	DUF905 family protein	-
CEQ27_RS16535	2987989..2988807	+	819	WP_001234716.1	DUF945 domain-containing protein	-
CEQ27_RS29975	2988807..2988980	+	174	WP_001183321.1	hypothetical protein	-
CEQ27_RS16545	2989146..2989619	+	474	WP_001387789.1	antirestriction protein	-
CEQ27_RS16550	2989635..2990111	+	477	WP_001186773.1	RadC family protein	-
CEQ27_RS16555	2990174..2990395	+	222	WP_000692300.1	DUF987 domain-containing protein	-
CEQ27_RS16560	2990558..2990935	+	378	WP_001390338.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CEQ27_RS16565	2990982..2991359	+	378	WP_001094437.1	TA system toxin CbtA family protein	Toxin
CEQ27_RS16570	2991356..2991844	+	489	WP_000761716.1	hypothetical protein	-
CEQ27_RS16575	2991864..2992061	+	198	WP_000772032.1	DUF957 domain-containing protein	-
CEQ27_RS16580	2992146..2992991	+	846	WP_001274561.1	DUF4942 domain-containing protein	-
CEQ27_RS16595	2993784..2994968	+	1185	WP_001172875.1	sugar efflux transporter	-
CEQ27_RS16600	2995079..2996002	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.90 Da
• Isoelectric Point: 7.3223

>T98616 WP_001094437.1 NZ_CP027394:2990982-2991359 [Escherichia coli O104:H4]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T98616 NZ_CP027394:2990982-2991359 [Escherichia coli O104:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13745.44 Da
• Isoelectric Point: 5.0463

>AT98616 WP_001390338.1 NZ_CP027394:2990558-2990935 [Escherichia coli O104:H4]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYAYLAVYPTPAEPAITV

Nucleotide

• Length: 378 bp

>AT98616 NZ_CP027394:2990558-2990935 [Escherichia coli O104:H4]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAGGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGCTTATCTGGCTGTTTATCCGACACCGGCAGAGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XS49

Antitoxin

• AlphaFold DB: A0A0E0XTQ1