Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2018961..2019615 Replicon chromosome
Accession NZ_CP027394
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag CEQ27_RS11315 Protein ID WP_000244772.1
Coordinates 2018961..2019368 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CEQ27_RS11320 Protein ID WP_000354046.1
Coordinates 2019349..2019615 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS11295 2014918..2016651 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
CEQ27_RS11300 2016657..2017367 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CEQ27_RS11305 2017392..2018288 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CEQ27_RS11310 2018400..2018921 + 522 WP_001055874.1 flavodoxin FldB -
CEQ27_RS11315 2018961..2019368 - 408 WP_000244772.1 protein YgfX Toxin
CEQ27_RS11320 2019349..2019615 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CEQ27_RS11325 2019858..2020838 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CEQ27_RS11330 2020916..2021575 - 660 WP_000250274.1 hemolysin III family protein -
CEQ27_RS11335 2021739..2022050 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CEQ27_RS11340 2022095..2023528 + 1434 WP_001387034.1 6-phospho-beta-glucosidase BglA -
CEQ27_RS11345 2023585..2024328 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T98613 WP_000244772.1 NZ_CP027394:c2019368-2018961 [Escherichia coli O104:H4]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98613 NZ_CP027394:c2019368-2018961 [Escherichia coli O104:H4]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98613 WP_000354046.1 NZ_CP027394:c2019615-2019349 [Escherichia coli O104:H4]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98613 NZ_CP027394:c2019615-2019349 [Escherichia coli O104:H4]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References