Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 60921..61446 Replicon plasmid unnamed2
Accession NZ_CP027392
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0E0Y871
Locus tag CEQ27_RS00425 Protein ID WP_001159872.1
Coordinates 61141..61446 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0E0Y6P9
Locus tag CEQ27_RS00420 Protein ID WP_000813638.1
Coordinates 60921..61139 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS00385 56105..56230 + 126 WP_032159539.1 hypothetical protein -
CEQ27_RS00390 56351..57091 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
CEQ27_RS00395 57370..58347 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
CEQ27_RS00405 59439..59588 + 150 Protein_68 transposase domain-containing protein -
CEQ27_RS00410 59671..60072 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin -
CEQ27_RS00415 60096..60326 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CEQ27_RS00420 60921..61139 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CEQ27_RS00425 61141..61446 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB Toxin
CEQ27_RS00430 61447..62253 + 807 WP_000016961.1 site-specific integrase -
CEQ27_RS00435 62432..63076 + 645 WP_001144032.1 ParA family protein -
CEQ27_RS00440 63163..63471 + 309 WP_000030204.1 molecular chaperone GroEL -
CEQ27_RS00445 63885..64865 + 981 WP_000688504.1 plasmid segregation protein ParM -
CEQ27_RS00450 64858..65274 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggD / aggC / aggB / aggA 1..75562 75562


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11722.51 Da        Isoelectric Point: 5.6919

>T98600 WP_001159872.1 NZ_CP027392:61141-61446 [Escherichia coli O104:H4]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHWENDIKNAINLMFWGI

Download         Length: 306 bp

>T98600 NZ_CP027392:61141-61446 [Escherichia coli O104:H4]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTAATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACTGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8344.29 Da        Isoelectric Point: 4.5203

>AT98600 WP_000813638.1 NZ_CP027392:60921-61139 [Escherichia coli O104:H4]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVAKFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98600 NZ_CP027392:60921-61139 [Escherichia coli O104:H4]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAAGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y871

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6P9

References