Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59671..60326 Replicon plasmid unnamed2
Accession NZ_CP027392
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name vagD Uniprot ID A0A2H3M3N3
Locus tag CEQ27_RS00410 Protein ID WP_001392130.1
Coordinates 59671..60072 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A0E0YAG4
Locus tag CEQ27_RS00415 Protein ID WP_001261288.1
Coordinates 60096..60326 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS00365 54809..55093 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein -
CEQ27_RS00370 55093..55368 + 276 WP_000421273.1 type II toxin-antitoxin system RelE/ParE family toxin -
CEQ27_RS00375 55374..55705 + 332 Protein_63 type II toxin-antitoxin system ParD family antitoxin -
CEQ27_RS00380 55821..55982 + 162 WP_001440647.1 type II toxin-antitoxin system RelE/ParE family toxin -
CEQ27_RS00385 56105..56230 + 126 WP_032159539.1 hypothetical protein -
CEQ27_RS00390 56351..57091 + 741 WP_001066951.1 tyrosine-type recombinase/integrase -
CEQ27_RS00395 57370..58347 - 978 WP_001387465.1 RepB family plasmid replication initiator protein -
CEQ27_RS00405 59439..59588 + 150 Protein_68 transposase domain-containing protein -
CEQ27_RS00410 59671..60072 - 402 WP_001392130.1 type II toxin-antitoxin system VapC family toxin Toxin
CEQ27_RS00415 60096..60326 - 231 WP_001261288.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CEQ27_RS00420 60921..61139 + 219 WP_000813638.1 type II toxin-antitoxin system antitoxin CcdA -
CEQ27_RS00425 61141..61446 + 306 WP_001159872.1 type II toxin-antitoxin system toxin CcdB -
CEQ27_RS00430 61447..62253 + 807 WP_000016961.1 site-specific integrase -
CEQ27_RS00435 62432..63076 + 645 WP_001144032.1 ParA family protein -
CEQ27_RS00440 63163..63471 + 309 WP_000030204.1 molecular chaperone GroEL -
CEQ27_RS00445 63885..64865 + 981 WP_000688504.1 plasmid segregation protein ParM -
CEQ27_RS00450 64858..65274 + 417 WP_001278815.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - aap/aspU / aggD / aggC / aggB / aggA 1..75562 75562


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14647.00 Da        Isoelectric Point: 6.7123

>T98599 WP_001392130.1 NZ_CP027392:c60072-59671 [Escherichia coli O104:H4]
MLDTNICSFIMREQPAAVIKRLEQAVLRNHRIVVSAITYAEMRFDATGPKASPRHIALVDAFCARLDAILPWDRAAVDAT
TEIKMALRLAETPIGPNNTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 402 bp

>T98599 NZ_CP027392:c60072-59671 [Escherichia coli O104:H4]
ATGCTCGACACGAATATCTGCTCTTTCATTATGCGTGAACAGCCCGCCGCAGTGATCAAACGTCTGGAGCAGGCGGTGCT
GCGTAACCACCGCATTGTCGTGTCGGCCATCACCTATGCGGAGATGCGTTTCGACGCCACCGGCCCGAAAGCCTCACCGC
GTCATATTGCGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATTCTGCCCTGGGACCGCGCTGCCGTGGACGCCACC
ACGGAGATTAAAATGGCACTGCGGCTGGCCGAGACGCCAATCGGCCCGAACAACACGGCGATTGCCGGGCACGCCATCGC
TGCCGGGGCGATACTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGACCTGGTGCTGGAAGACTGGGTGAAGT
AA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8684.76 Da        Isoelectric Point: 4.3654

>AT98599 WP_001261288.1 NZ_CP027392:c60326-60096 [Escherichia coli O104:H4]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWSSFAALEKADADFMAEREDIVSDEGRFDL

Download         Length: 231 bp

>AT98599 NZ_CP027392:c60326-60096 [Escherichia coli O104:H4]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTTGAGATCGTCCGCGAAGGAGACAGCATTATATTGCGCCCGGTCCGGCCGACCTGGAGCTCGTTTGCGGCACTCG
AAAAGGCCGACGCAGACTTTATGGCGGAGCGTGAGGACATTGTCAGCGACGAAGGAAGATTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H3M3N3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0YAG4

References