Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 54809..55368 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP027392 | ||
| Organism | Escherichia coli O104:H4 strain FDAARGOS_349 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G4VUF8 |
| Locus tag | CEQ27_RS00370 | Protein ID | WP_000421273.1 |
| Coordinates | 55093..55368 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0SRR3 |
| Locus tag | CEQ27_RS00365 | Protein ID | WP_001387467.1 |
| Coordinates | 54809..55093 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CEQ27_RS00310 | 49848..50525 | - | 678 | WP_001339397.1 | IS66 family insertion sequence hypothetical protein | - |
| CEQ27_RS00315 | 50590..50802 | + | 213 | Protein_55 | ANR family transcriptional regulator | - |
| CEQ27_RS00330 | 51253..52233 | - | 981 | WP_000019440.1 | IS5-like element ISKpn26 family transposase | - |
| CEQ27_RS00335 | 52514..52768 | + | 255 | WP_000083850.1 | replication regulatory protein RepA | - |
| CEQ27_RS29790 | 52875..53009 | + | 135 | Protein_58 | protein CopA/IncA | - |
| CEQ27_RS00345 | 53006..53080 | + | 75 | WP_014966221.1 | RepA leader peptide Tap | - |
| CEQ27_RS00350 | 53073..53901 | + | 829 | Protein_60 | incFII family plasmid replication initiator RepA | - |
| CEQ27_RS00365 | 54809..55093 | + | 285 | WP_001387467.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| CEQ27_RS00370 | 55093..55368 | + | 276 | WP_000421273.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| CEQ27_RS00375 | 55374..55705 | + | 332 | Protein_63 | type II toxin-antitoxin system ParD family antitoxin | - |
| CEQ27_RS00380 | 55821..55982 | + | 162 | WP_001440647.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| CEQ27_RS00385 | 56105..56230 | + | 126 | WP_032159539.1 | hypothetical protein | - |
| CEQ27_RS00390 | 56351..57091 | + | 741 | WP_001066951.1 | tyrosine-type recombinase/integrase | - |
| CEQ27_RS00395 | 57370..58347 | - | 978 | WP_001387465.1 | RepB family plasmid replication initiator protein | - |
| CEQ27_RS00405 | 59439..59588 | + | 150 | Protein_68 | transposase domain-containing protein | - |
| CEQ27_RS00410 | 59671..60072 | - | 402 | WP_001392130.1 | type II toxin-antitoxin system VapC family toxin | - |
| CEQ27_RS00415 | 60096..60326 | - | 231 | WP_001261288.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | aap/aspU / aggD / aggC / aggB / aggA | 1..75562 | 75562 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10765.45 Da Isoelectric Point: 9.9324
>T98598 WP_000421273.1 NZ_CP027392:55093-55368 [Escherichia coli O104:H4]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T98598 NZ_CP027392:55093-55368 [Escherichia coli O104:H4]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10460.87 Da Isoelectric Point: 5.6558
>AT98598 WP_001387467.1 NZ_CP027392:54809-55093 [Escherichia coli O104:H4]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR
Download Length: 285 bp
>AT98598 NZ_CP027392:54809-55093 [Escherichia coli O104:H4]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y6H1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0Y880 |