Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14697..15340 Replicon plasmid unnamed
Accession NZ_CP027391
Organism Escherichia coli strain 2015C-4944

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag AX062_RS31800 Protein ID WP_001044768.1
Coordinates 14924..15340 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag AX062_RS31795 Protein ID WP_001261287.1
Coordinates 14697..14927 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX062_RS31750 10562..12001 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
AX062_RS31755 12068..12211 + 144 Protein_8 transcriptional regulator -
AX062_RS31760 12215..12406 + 192 Protein_9 lipid II-degrading bacteriocin -
AX062_RS31765 12414..12767 - 354 WP_000864810.1 colicin M immunity protein -
AX062_RS31770 12940..13722 - 783 WP_001164205.1 site-specific integrase -
AX062_RS31775 13724..14137 - 414 WP_000465041.1 hypothetical protein -
AX062_RS31795 14697..14927 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AX062_RS31800 14924..15340 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
AX062_RS31805 15502..16047 - 546 WP_001165114.1 hypothetical protein -
AX062_RS31810 16115..17271 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -
AX062_RS32980 17312..17461 - 150 WP_000955366.1 hypothetical protein -
AX062_RS31815 18076..18936 - 861 WP_000704534.1 alpha/beta hydrolase -
AX062_RS31820 19064..19450 + 387 WP_001066949.1 tyrosine-type recombinase/integrase -
AX062_RS31825 19504..20178 + 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - hlyC / hlyA / hlyB / hlyD / toxB / espP 1..98724 98724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T98597 WP_001044768.1 NZ_CP027391:14924-15340 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T98597 NZ_CP027391:14924-15340 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT98597 WP_001261287.1 NZ_CP027391:14697-14927 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT98597 NZ_CP027391:14697-14927 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References