Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3535673..3536170 Replicon chromosome
Accession NZ_CP027390
Organism Escherichia coli strain 2015C-4944

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AX062_RS19780 Protein ID WP_001173343.1
Coordinates 3535673..3535846 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AX062_RS19785 Protein ID WP_000398619.1
Coordinates 3535874..3536170 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX062_RS19770 3532343..3533800 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
AX062_RS19775 3534037..3535554 + 1518 WP_001295074.1 lysine--tRNA ligase -
AX062_RS19780 3535673..3535846 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AX062_RS19785 3535874..3536170 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AX062_RS19795 3536397..3536669 - 273 WP_000405647.1 N-acetyltransferase -
AX062_RS19800 3536681..3536911 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
AX062_RS19805 3537092..3538723 + 1632 WP_001216487.1 sensor histidine kinase -
AX062_RS19810 3538720..3539439 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espL2 / nleB1 / nleE / efa1 / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae / cesT / tir / map / cesF / espH / sepQ/escQ / escO / escN / escV / cesL / sepZ/espZ / escI / escJ / sepD / escC / cesD / etgA / escU / escT / escS / escR / escL / escK / cesAB / escE / espG 3470648..3536170 65522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98590 WP_001173343.1 NZ_CP027390:c3535846-3535673 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98590 NZ_CP027390:c3535846-3535673 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT98590 WP_000398619.1 NZ_CP027390:c3536170-3535874 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98590 NZ_CP027390:c3536170-3535874 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References