Detailed information of TA system TA098588

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3297139..3297971
• Replicon: chromosome
• Accession: NZ_CP027390
• Organism: Escherichia coli strain 2015C-4944

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: AX062_RS18495
• Protein ID: WP_000854753.1
• Coordinates: 3297139..3297513 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: AX062_RS18500
• Protein ID: WP_001315620.1
• Coordinates: 3297603..3297971 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX062_RS18450	3292354..3293460	+	1107	WP_001341302.1	N-acetylneuraminate epimerase	-
AX062_RS18455	3293525..3294505	+	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
AX062_RS18460	3294513..3295163	-	651	WP_001037966.1	HNH endonuclease	-
AX062_RS32900	3296208..3296360	-	153	WP_001280445.1	hypothetical protein	-
AX062_RS18485	3296445..3296642	-	198	WP_000839281.1	DUF957 domain-containing protein	-
AX062_RS18490	3296654..3297142	-	489	WP_000777547.1	hypothetical protein	-
AX062_RS18495	3297139..3297513	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
AX062_RS18500	3297603..3297971	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AX062_RS18505	3298134..3298355	-	222	WP_000692345.1	DUF987 domain-containing protein	-
AX062_RS18510	3298418..3298894	-	477	WP_001186774.1	RadC family protein	-
AX062_RS18515	3298910..3299395	-	486	WP_000849588.1	antirestriction protein	-
AX062_RS18520	3299450..3300268	-	819	WP_001234622.1	DUF945 domain-containing protein	-
AX062_RS18530	3300368..3300601	-	234	WP_001119729.1	DUF905 family protein	-
AX062_RS18535	3300680..3301135	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimE / fimB	3288486..3306548	18062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T98588 WP_000854753.1 NZ_CP027390:c3297513-3297139 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T98588 NZ_CP027390:c3297513-3297139 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT98588 WP_001315620.1 NZ_CP027390:c3297971-3297603 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT98588 NZ_CP027390:c3297971-3297603 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5