Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 603571..604042 Replicon chromosome
Accession NZ_CP027390
Organism Escherichia coli strain 2015C-4944

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag AX062_RS03235 Protein ID WP_001448501.1
Coordinates 603571..603849 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag AX062_RS03240 Protein ID WP_001302048.1
Coordinates 603851..604042 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX062_RS03205 598824..599228 - 405 Protein_593 recombinase RecT -
AX062_RS03210 599221..601872 - 2652 WP_000105101.1 exodeoxyribonuclease VIII -
AX062_RS03215 601971..602246 - 276 WP_001307773.1 hypothetical protein -
AX062_RS03220 602320..602496 - 177 WP_000245534.1 hypothetical protein -
AX062_RS03225 602490..602711 - 222 WP_000560218.1 killing protein KilR -
AX062_RS03230 603132..603284 - 153 WP_001427316.1 DUF1391 family protein -
AX062_RS03235 603571..603849 - 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AX062_RS03240 603851..604042 - 192 WP_001302048.1 hypothetical protein Antitoxin
AX062_RS03245 604063..604434 - 372 WP_001169687.1 hypothetical protein -
AX062_RS03250 604532..604834 + 303 WP_000172738.1 transcriptional regulator -
AX062_RS03255 604831..605256 + 426 WP_000693943.1 Rha family transcriptional regulator -
AX062_RS03260 605279..606241 + 963 WP_001444941.1 helix-turn-helix domain-containing protein -
AX062_RS03265 606248..606989 + 742 Protein_605 ATP-binding protein -
AX062_RS03270 607015..607776 + 762 WP_000450872.1 DUF1627 domain-containing protein -
AX062_RS03275 607809..608090 + 282 WP_000603384.1 DNA-binding protein -
AX062_RS03280 608087..608314 + 228 WP_000699809.1 hypothetical protein -
AX062_RS03285 608307..608618 + 312 WP_001289673.1 hypothetical protein -
AX062_RS03290 608746..608964 + 219 WP_000683609.1 DUF4014 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 589424..661668 72244


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T98573 WP_001448501.1 NZ_CP027390:c603849-603571 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98573 NZ_CP027390:c603849-603571 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98573 WP_001302048.1 NZ_CP027390:c604042-603851 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98573 NZ_CP027390:c604042-603851 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References