Detailed information of TA system TA098558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 32378..32913
• Replicon: plasmid unnamed
• Accession: NZ_CP027389
• Organism: Escherichia coli strain 2011C-4251

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6P70_RS29225
• Protein ID: WP_001270421.1
• Coordinates: 32378..32665 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: C6P70_RS29230
• Protein ID: WP_001132895.1
• Coordinates: 32662..32913 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P70_RS29185	27781..27960	-	180	WP_000526860.1	hypothetical protein	-
C6P70_RS29190	28163..28537	+	375	WP_001368887.1	hypothetical protein	-
C6P70_RS29195	28848..29009	-	162	Protein_23	epoxyqueuosine reductase QueH	-
C6P70_RS29200	29060..29200	+	141	Protein_24	serine/threonine protein kinase	-
C6P70_RS29205	29324..30064	+	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
C6P70_RS29210	30348..31325	-	978	WP_000361617.1	RepB family plasmid replication initiator protein	-
C6P70_RS29220	31971..32376	-	406	Protein_27	DDE-type integrase/transposase/recombinase	-
C6P70_RS29225	32378..32665	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6P70_RS29230	32662..32913	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6P70_RS29855	33087..33260	-	174	WP_001435278.1	hypothetical protein	-
C6P70_RS29240	33631..34836	+	1206	WP_000817643.1	AAA family ATPase	-
C6P70_RS29245	34833..35795	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
C6P70_RS29250	35936..37098	-	1163	Protein_33	DUF4113 domain-containing protein	-
C6P70_RS29255	36979..37396	+	418	Protein_34	DUF1281 domain-containing protein	-
C6P70_RS29260	37400..37705	+	306	WP_032162675.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyB / hlyA / hlyC / stcE / exeE / exeG	1..68062	68062

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T98558 WP_001270421.1 NZ_CP027389:c32665-32378 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T98558 NZ_CP027389:c32665-32378 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT98558 WP_001132895.1 NZ_CP027389:c32913-32662 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT98558 NZ_CP027389:c32913-32662 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTTTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6