Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 4285767..4286238 Replicon chromosome
Accession NZ_CP027387
Organism Escherichia coli strain 2014C-3057

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag C6P73_RS22730 Protein ID WP_001448501.1
Coordinates 4285767..4286045 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6P73_RS22735 Protein ID WP_001302048.1
Coordinates 4286047..4286238 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P73_RS22705 4281417..4284068 - 2652 WP_000105101.1 exodeoxyribonuclease VIII -
C6P73_RS22710 4284167..4284442 - 276 WP_001307773.1 hypothetical protein -
C6P73_RS22715 4284516..4284692 - 177 WP_000245534.1 hypothetical protein -
C6P73_RS22720 4284686..4284907 - 222 WP_000560218.1 killing protein KilR -
C6P73_RS22725 4285328..4285480 - 153 WP_001427316.1 DUF1391 family protein -
C6P73_RS22730 4285767..4286045 - 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6P73_RS22735 4286047..4286238 - 192 WP_001302048.1 hypothetical protein Antitoxin
C6P73_RS22740 4286259..4286630 - 372 WP_001169687.1 hypothetical protein -
C6P73_RS22745 4286728..4287030 + 303 WP_000172738.1 transcriptional regulator -
C6P73_RS22750 4287027..4287452 + 426 WP_000693943.1 Rha family transcriptional regulator -
C6P73_RS22755 4287475..4288437 + 963 WP_001444941.1 helix-turn-helix domain-containing protein -
C6P73_RS22760 4288444..4289185 + 742 Protein_4101 ATP-binding protein -
C6P73_RS22765 4289211..4289972 + 762 WP_000450872.1 DUF1627 domain-containing protein -
C6P73_RS22770 4290005..4290286 + 282 WP_000603384.1 DNA-binding protein -
C6P73_RS22775 4290283..4290510 + 228 WP_000699809.1 hypothetical protein -
C6P73_RS22780 4290503..4290814 + 312 WP_001289673.1 hypothetical protein -
C6P73_RS22785 4290942..4291160 + 219 WP_000683609.1 DUF4014 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 4272887..4343866 70979


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T98517 WP_001448501.1 NZ_CP027387:c4286045-4285767 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98517 NZ_CP027387:c4286045-4285767 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98517 WP_001302048.1 NZ_CP027387:c4286238-4286047 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98517 NZ_CP027387:c4286238-4286047 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References