Detailed information of TA system TA098502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3412107..3412732
• Replicon: chromosome
• Accession: NZ_CP027387
• Organism: Escherichia coli strain 2014C-3057

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6P73_RS18035
• Protein ID: WP_000911330.1
• Coordinates: 3412334..3412732 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6P73_RS18030
• Protein ID: WP_000450524.1
• Coordinates: 3412107..3412334 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P73_RS18005	3407910..3408380	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6P73_RS18010	3408380..3408952	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6P73_RS18015	3409098..3409976	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6P73_RS18020	3409993..3411027	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6P73_RS18025	3411240..3411953	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6P73_RS18030	3412107..3412334	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6P73_RS18035	3412334..3412732	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6P73_RS18040	3412879..3413742	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6P73_RS18045	3413757..3415772	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6P73_RS18050	3415846..3416544	+	699	WP_000679812.1	esterase	-
C6P73_RS18055	3416654..3416854	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98502 WP_000911330.1 NZ_CP027387:3412334-3412732 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98502 NZ_CP027387:3412334-3412732 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98502 WP_000450524.1 NZ_CP027387:3412107-3412334 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98502 NZ_CP027387:3412107-3412334 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33