Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1305546..1306378 Replicon chromosome
Accession NZ_CP027387
Organism Escherichia coli strain 2014C-3057

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag C6P73_RS07105 Protein ID WP_000854753.1
Coordinates 1305546..1305920 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID V0ULY5
Locus tag C6P73_RS07110 Protein ID WP_001315620.1
Coordinates 1306010..1306378 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P73_RS07060 1300761..1301867 + 1107 WP_001341302.1 N-acetylneuraminate epimerase -
C6P73_RS07065 1301932..1302912 + 981 WP_000991415.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
C6P73_RS07070 1302920..1303570 - 651 WP_001037966.1 HNH endonuclease -
C6P73_RS31480 1304615..1304767 - 153 WP_001280445.1 hypothetical protein -
C6P73_RS07095 1304852..1305049 - 198 WP_000839281.1 DUF957 domain-containing protein -
C6P73_RS07100 1305061..1305549 - 489 WP_000777547.1 hypothetical protein -
C6P73_RS07105 1305546..1305920 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
C6P73_RS07110 1306010..1306378 - 369 WP_001315620.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
C6P73_RS07115 1306541..1306762 - 222 WP_000692345.1 DUF987 domain-containing protein -
C6P73_RS07120 1306825..1307301 - 477 WP_001186774.1 RadC family protein -
C6P73_RS07125 1307317..1307802 - 486 WP_000849588.1 antirestriction protein -
C6P73_RS07130 1307857..1308675 - 819 WP_001234622.1 DUF945 domain-containing protein -
C6P73_RS07140 1308775..1309008 - 234 WP_001119729.1 DUF905 family protein -
C6P73_RS07145 1309087..1309542 - 456 WP_000581504.1 hypothetical protein -
C6P73_RS07150 1309618..1310667 - 1050 Protein_1250 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimE / fimB 1296893..1325314 28421


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T98494 WP_000854753.1 NZ_CP027387:c1305920-1305546 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T98494 NZ_CP027387:c1305920-1305546 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13474.20 Da        Isoelectric Point: 6.2050

>AT98494 WP_001315620.1 NZ_CP027387:c1306378-1306010 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT98494 NZ_CP027387:c1306378-1306010 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB V0ULY5

References