Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18506..19149 Replicon plasmid unnamed
Accession NZ_CP027386
Organism Escherichia coli strain 2014C-3057

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6P73_RS00115 Protein ID WP_001044768.1
Coordinates 18733..19149 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6P73_RS00110 Protein ID WP_001261287.1
Coordinates 18506..18736 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P73_RS00065 14369..15808 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
C6P73_RS00070 15875..16018 + 144 Protein_11 transcriptional regulator -
C6P73_RS00075 16022..16213 + 192 Protein_12 lipid II-degrading bacteriocin -
C6P73_RS00080 16221..16575 - 355 Protein_13 colicin M immunity protein -
C6P73_RS00085 16748..17530 - 783 WP_001164205.1 site-specific integrase -
C6P73_RS00090 17532..17945 - 414 WP_000465041.1 hypothetical protein -
C6P73_RS00110 18506..18736 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6P73_RS00115 18733..19149 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6P73_RS00120 19311..19856 - 546 WP_001165114.1 hypothetical protein -
C6P73_RS00125 19924..21080 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -
C6P73_RS30965 21121..21396 - 276 WP_157776129.1 hypothetical protein -
C6P73_RS00130 21885..22745 - 861 WP_000704534.1 alpha/beta hydrolase -
C6P73_RS00135 22873..23259 + 387 WP_106875054.1 tyrosine-type recombinase/integrase -
C6P73_RS00140 23313..23987 + 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyC / hlyA / hlyB / hlyB / hlyD / toxB 1..54452 54452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T98490 WP_001044768.1 NZ_CP027386:18733-19149 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T98490 NZ_CP027386:18733-19149 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT98490 WP_001261287.1 NZ_CP027386:18506-18736 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT98490 NZ_CP027386:18506-18736 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References