Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2925125..2925596 Replicon chromosome
Accession NZ_CP027380
Organism Escherichia coli strain 2013C-3250

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag AYN27_RS16165 Protein ID WP_001303511.1
Coordinates 2925318..2925596 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag AYN27_RS16160 Protein ID WP_001302048.1
Coordinates 2925125..2925316 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN27_RS16115 2920598..2920870 - 273 WP_012817871.1 hypothetical protein -
AYN27_RS16125 2921038..2921178 - 141 WP_072058819.1 type I toxin-antitoxin system Hok family toxin -
AYN27_RS16130 2921216..2922429 + 1214 WP_162829202.1 IS3 family transposase -
AYN27_RS16135 2922469..2922885 - 417 WP_023981635.1 DUF977 family protein -
AYN27_RS16140 2922926..2923888 - 963 WP_000095671.1 helix-turn-helix domain-containing protein -
AYN27_RS16145 2923911..2924336 - 426 WP_000693943.1 Rha family transcriptional regulator -
AYN27_RS16150 2924333..2924635 - 303 WP_001444689.1 transcriptional regulator -
AYN27_RS16155 2924733..2925104 + 372 WP_001169687.1 hypothetical protein -
AYN27_RS16160 2925125..2925316 + 192 WP_001302048.1 hypothetical protein Antitoxin
AYN27_RS16165 2925318..2925596 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYN27_RS16175 2925892..2926281 + 390 WP_001447517.1 hypothetical protein -
AYN27_RS16180 2926322..2926540 + 219 WP_001171930.1 hypothetical protein -
AYN27_RS16190 2927024..2927212 + 189 WP_000449192.1 cell division inhibition protein DicB -
AYN27_RS16195 2927209..2927400 + 192 WP_001090200.1 DUF1482 family protein -
AYN27_RS16200 2927493..2929958 + 2466 WP_000048442.1 exonuclease -
AYN27_RS16205 2930020..2930289 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espN / espX7/nleL 2877285..2938545 61260


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T98477 WP_001303511.1 NZ_CP027380:2925318-2925596 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98477 NZ_CP027380:2925318-2925596 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATTCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98477 WP_001302048.1 NZ_CP027380:2925125-2925316 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98477 NZ_CP027380:2925125-2925316 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References