Detailed information of TA system TA098476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2509485..2510123
• Replicon: chromosome
• Accession: NZ_CP027380
• Organism: Escherichia coli strain 2013C-3250

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AYN27_RS13800
• Protein ID: WP_000813794.1
• Coordinates: 2509947..2510123 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AYN27_RS13795
• Protein ID: WP_001270286.1
• Coordinates: 2509485..2509901 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN27_RS13775	2504637..2505578	-	942	WP_001251315.1	ABC transporter permease	-
AYN27_RS13780	2505579..2506592	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AYN27_RS13785	2506610..2507755	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
AYN27_RS13790	2508000..2509406	-	1407	WP_000760637.1	PLP-dependent aminotransferase family protein	-
AYN27_RS13795	2509485..2509901	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AYN27_RS13800	2509947..2510123	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AYN27_RS13805	2510345..2510575	+	231	WP_000494244.1	YncJ family protein	-
AYN27_RS13810	2510667..2512628	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AYN27_RS13815	2512701..2513237	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AYN27_RS13820	2513290..2514498	+	1209	WP_071532666.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2514538..2515686	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T98476 WP_000813794.1 NZ_CP027380:c2510123-2509947 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T98476 NZ_CP027380:c2510123-2509947 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT98476 WP_001270286.1 NZ_CP027380:c2509901-2509485 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT98476 NZ_CP027380:c2509901-2509485 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB