Detailed information of TA system TA098465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1170421..1171046
• Replicon: chromosome
• Accession: NZ_CP027380
• Organism: Escherichia coli strain 2013C-3250

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AYN27_RS06425
• Protein ID: WP_000911330.1
• Coordinates: 1170648..1171046 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AYN27_RS06420
• Protein ID: WP_000450524.1
• Coordinates: 1170421..1170648 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN27_RS06395	1166206..1166676	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
AYN27_RS06400	1166676..1167266	-	591	WP_106914110.1	glycine cleavage system transcriptional repressor	-
AYN27_RS06405	1167412..1168290	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AYN27_RS06410	1168307..1169341	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AYN27_RS06415	1169554..1170267	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AYN27_RS06420	1170421..1170648	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AYN27_RS06425	1170648..1171046	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AYN27_RS06430	1171193..1172056	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
AYN27_RS06435	1172071..1174086	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
AYN27_RS06440	1174160..1174858	+	699	WP_000679812.1	esterase	-
AYN27_RS06445	1174968..1175168	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98465 WP_000911330.1 NZ_CP027380:1170648-1171046 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98465 NZ_CP027380:1170648-1171046 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98465 WP_000450524.1 NZ_CP027380:1170421-1170648 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98465 NZ_CP027380:1170421-1170648 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33