98458

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 43626..44151 Replicon plasmid unnamed2
Accession NZ_CP027375
Organism Escherichia coli strain 05-3629

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6P66_RS26390 Protein ID WP_001159871.1
Coordinates 43626..43931 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6P66_RS26395 Protein ID WP_000813639.1
Coordinates 43933..44151 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P66_RS26355 38893..40163 + 1271 Protein_55 translesion error-prone DNA polymerase V subunit UmuC -
C6P66_RS26365 40168..40560 - 393 WP_000340836.1 plasmid partitioning/stability family protein -
C6P66_RS26370 40565..41536 - 972 WP_001103695.1 hypothetical protein -
C6P66_RS26375 41765..42409 + 645 WP_000633912.1 AAA family ATPase -
C6P66_RS26380 42403..42678 + 276 WP_000239527.1 hypothetical protein -
C6P66_RS26385 42816..43625 - 810 WP_052892429.1 site-specific integrase -
C6P66_RS26390 43626..43931 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6P66_RS26395 43933..44151 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6P66_RS26400 44747..44995 + 249 WP_052892430.1 hypothetical protein -
C6P66_RS26405 44992..45582 + 591 WP_052892431.1 hypothetical protein -
C6P66_RS26410 45600..45947 - 348 WP_000142427.1 hypothetical protein -
C6P66_RS26420 46430..46801 - 372 WP_052892432.1 colicin -
C6P66_RS26425 46861..47592 - 732 WP_000850414.1 complement resistance protein TraT -
C6P66_RS26435 47864..47974 - 111 Protein_68 IS481 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..118863 118863


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T98458 WP_001159871.1 NZ_CP027375:c43931-43626 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98458 NZ_CP027375:c43931-43626 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT98458 WP_000813639.1 NZ_CP027375:c44151-43933 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98458 NZ_CP027375:c44151-43933 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References