Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4630452..4631090 Replicon chromosome
Accession NZ_CP027373
Organism Escherichia coli strain 05-3629

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6P66_RS23900 Protein ID WP_000813794.1
Coordinates 4630914..4631090 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6P66_RS23895 Protein ID WP_001270286.1
Coordinates 4630452..4630868 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P66_RS23875 4625604..4626545 - 942 WP_001251313.1 ABC transporter permease -
C6P66_RS23880 4626546..4627559 - 1014 WP_001542893.1 ABC transporter ATP-binding protein -
C6P66_RS23885 4627577..4628722 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
C6P66_RS23890 4628967..4630373 - 1407 WP_000760663.1 PLP-dependent aminotransferase family protein -
C6P66_RS23895 4630452..4630868 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6P66_RS23900 4630914..4631090 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6P66_RS23905 4631312..4631542 + 231 WP_000494244.1 YncJ family protein -
C6P66_RS23910 4631634..4633595 - 1962 WP_001442195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6P66_RS23915 4633668..4634204 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C6P66_RS23920 4634257..4635471 + 1215 WP_071609973.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4635511..4636659 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T98448 WP_000813794.1 NZ_CP027373:c4631090-4630914 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T98448 NZ_CP027373:c4631090-4630914 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT98448 WP_001270286.1 NZ_CP027373:c4630868-4630452 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT98448 NZ_CP027373:c4630868-4630452 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References