Detailed information of TA system TA098433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2383299..2384099
• Replicon: chromosome
• Accession: NZ_CP027373
• Organism: Escherichia coli strain 05-3629

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: C6P66_RS12265
• Protein ID: WP_000342449.1
• Coordinates: 2383572..2384099 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: C6P66_RS12260
• Protein ID: WP_001277108.1
• Coordinates: 2383299..2383565 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P66_RS12240	2378957..2379625	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
C6P66_RS12245	2379618..2380676	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
C6P66_RS12250	2380921..2381775	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
C6P66_RS12255	2382046..2383149	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
C6P66_RS12260	2383299..2383565	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
C6P66_RS12265	2383572..2384099	+	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
C6P66_RS12270	2384096..2384479	-	384	WP_000778795.1	aspartate 1-decarboxylase autocleavage activator PanM	-
C6P66_RS12275	2384903..2386012	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
C6P66_RS12280	2386060..2386986	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
C6P66_RS12285	2386983..2388260	+	1278	WP_000803771.1	branched chain amino acid ABC transporter permease LivM	-
C6P66_RS12290	2388257..2389024	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T98433 WP_000342449.1 NZ_CP027373:2383572-2384099 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T98433 NZ_CP027373:2383572-2384099 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT98433 WP_001277108.1 NZ_CP027373:2383299-2383565 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT98433 NZ_CP027373:2383299-2383565 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24