Detailed information of TA system TA098427

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1010244..1010933
• Replicon: chromosome
• Accession: NZ_CP027373
• Organism: Escherichia coli strain 05-3629

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: C6P66_RS05390
• Protein ID: WP_001460496.1
• Coordinates: 1010244..1010582 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: C6P66_RS05395
• Protein ID: WP_001460495.1
• Coordinates: 1010607..1010933 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P66_RS05370	1005334..1007532	+	2199	WP_106916210.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
C6P66_RS05375	1007542..1008498	+	957	WP_000121356.1	molybdenum cofactor insertion chaperone PaoD	-
C6P66_RS05380	1008549..1008887	+	339	Protein_969	LysR family transcriptional regulator	-
C6P66_RS05385	1009246..1010097	-	852	WP_097447682.1	hypothetical protein	-
C6P66_RS05390	1010244..1010582	-	339	WP_001460496.1	TA system toxin CbtA family protein	Toxin
C6P66_RS05395	1010607..1010933	-	327	WP_001460495.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6P66_RS05400	1010976..1011455	-	480	WP_097447683.1	DNA repair protein RadC	-
C6P66_RS05405	1011468..1011914	-	447	WP_050561233.1	antirestriction protein	-
C6P66_RS05410	1011993..1012811	-	819	WP_097447684.1	DUF945 domain-containing protein	-
C6P66_RS05415	1013274..1013897	+	624	WP_001461983.1	inovirus Gp2 family protein	-
C6P66_RS05420	1014016..1014231	+	216	WP_001461982.1	AlpA family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagW/ecpD / yagV/ecpE	982692..1025297	42605

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12771.67 Da
• Isoelectric Point: 8.5253

>T98427 WP_001460496.1 NZ_CP027373:c1010582-1010244 [Escherichia coli]
MKTLPANQRVAKPCPPPVLVWQTLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLADAVNFLVEKYELVRIDRRGFN
CQEQSPYLRAVDILRARQATGLLRQSHHPSAR

Nucleotide

• Length: 339 bp

>T98427 NZ_CP027373:c1010582-1010244 [Escherichia coli]
ATGAAAACTCTTCCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTACTTGTCTGGCAAACGCTACTTACACG
TCTGCTGGAGCAACACTATGGTCTGACGCTGAACGACACGCCGTTCAGTGATGAAACCGTTATTCAGGAGCATATCAATG
CCGGTATTACGCTGGCTGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGAATCGATCGCAGAGGGTTTAAC
TGTCAGGAGCAGTCACCTTACCTTCGGGCGGTAGATATTTTACGGGCCCGACAGGCAACCGGCCTCTTGCGGCAAAGCCA
TCATCCTTCGGCACGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12552.27 Da
• Isoelectric Point: 6.3682

>AT98427 WP_001460495.1 NZ_CP027373:c1010933-1010607 [Escherichia coli]
MEKHDTPEWGLRCDVMPRFSARLVQKNNRLHYLADRAGITGAFKEEECHQLNSAFPHFIEQMELMLLSGELNPRHAHRVT
LYHDELTCEADTLGSCGYVYMAIYPTQR

Nucleotide

• Length: 327 bp

>AT98427 NZ_CP027373:c1010933-1010607 [Escherichia coli]
ATGGAAAAACACGATACCCCTGAATGGGGCCTTCGGTGTGATGTAATGCCTCGCTTTAGTGCAAGGCTGGTACAGAAAAA
CAACCGGCTGCATTATTTAGCTGATCGCGCCGGGATAACTGGCGCTTTCAAAGAAGAAGAGTGCCACCAACTGAATAGCG
CATTTCCGCACTTTATAGAACAGATGGAATTGATGCTGCTTTCTGGTGAGCTTAACCCCCGCCATGCACACCGCGTCACT
CTGTACCATGACGAACTGACCTGTGAAGCGGATACACTTGGTAGCTGTGGCTACGTTTATATGGCGATTTATCCCACTCA
ACGTTAA