Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 110941..111412 Replicon chromosome
Accession NZ_CP027373
Organism Escherichia coli strain 05-3629

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C6P66_RS00735 Protein ID WP_001303511.1
Coordinates 111134..111412 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6P66_RS00730 Protein ID WP_001302048.1
Coordinates 110941..111132 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P66_RS27080 106309..106485 - 177 WP_000753060.1 hypothetical protein -
C6P66_RS00685 106478..106660 - 183 WP_032284905.1 hypothetical protein -
C6P66_RS00690 106808..107113 - 306 WP_072095369.1 DUF4406 domain-containing protein -
C6P66_RS00695 107110..107391 - 282 WP_072094476.1 DNA-binding protein -
C6P66_RS00700 107424..108140 - 717 WP_050877165.1 DUF1627 domain-containing protein -
C6P66_RS00705 108174..108716 - 543 WP_072130322.1 replication protein -
C6P66_RS26845 108628..109659 - 1032 WP_028985607.1 phage replisome organiser -
C6P66_RS00715 109728..110153 - 426 WP_000693925.1 Rha family transcriptional regulator -
C6P66_RS00720 110150..110452 - 303 WP_032279888.1 transcriptional regulator -
C6P66_RS00725 110549..110920 + 372 WP_001169685.1 hypothetical protein -
C6P66_RS00730 110941..111132 + 192 WP_001302048.1 hypothetical protein Antitoxin
C6P66_RS00735 111134..111412 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6P66_RS00740 111703..111855 + 153 WP_000367379.1 DUF1391 family protein -
C6P66_RS00745 111969..112484 - 516 WP_032284904.1 hypothetical protein -
C6P66_RS00760 113013..113201 + 189 WP_000450222.1 cell division inhibitor -
C6P66_RS00765 113198..113386 + 189 WP_000092784.1 DUF1482 family protein -
C6P66_RS00770 113479..115923 + 2445 WP_032284903.1 exonuclease -
C6P66_RS00775 115985..116254 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - stx2B / stx2A 53196..124510 71314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T98425 WP_001303511.1 NZ_CP027373:111134-111412 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98425 NZ_CP027373:111134-111412 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCAGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTGGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98425 WP_001302048.1 NZ_CP027373:110941-111132 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98425 NZ_CP027373:110941-111132 [Escherichia coli]
ATGAATAGAGCCCTTTCACCGATGGTTTCTGAGTTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References