Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3760477..3761108 Replicon chromosome
Accession NZ_CP027371
Organism Escherichia coli strain 2015C-3905

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6P67_RS19725 Protein ID WP_001259385.1
Coordinates 3760833..3761108 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag C6P67_RS19720 Protein ID WP_000593554.1
Coordinates 3760477..3760836 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P67_RS19690 3755589..3756062 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
C6P67_RS19695 3756059..3756340 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
C6P67_RS19700 3756417..3757775 + 1359 Protein_3590 PTS galactitol transporter subunit IIC -
C6P67_RS19705 3757768..3759276 + 1509 WP_001298718.1 carbohydrate kinase -
C6P67_RS19710 3759266..3759535 + 270 WP_000933060.1 HPr family phosphocarrier protein -
C6P67_RS19715 3759567..3760427 + 861 WP_001131783.1 class II aldolase -
C6P67_RS19720 3760477..3760836 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6P67_RS19725 3760833..3761108 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6P67_RS19730 3761181..3762305 - 1125 WP_001314210.1 ABC transporter permease -
C6P67_RS19735 3762305..3765040 - 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
C6P67_RS19740 3765037..3766104 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T98420 WP_001259385.1 NZ_CP027371:c3761108-3760833 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T98420 NZ_CP027371:c3761108-3760833 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT98420 WP_000593554.1 NZ_CP027371:c3760836-3760477 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT98420 NZ_CP027371:c3760836-3760477 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References