Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3098966..3099620 Replicon chromosome
Accession NZ_CP027371
Organism Escherichia coli strain 2015C-3905

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag C6P67_RS16160 Protein ID WP_000244772.1
Coordinates 3098966..3099373 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6P67_RS16165 Protein ID WP_000354046.1
Coordinates 3099354..3099620 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P67_RS16140 3094923..3096656 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
C6P67_RS16145 3096662..3097372 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6P67_RS16150 3097397..3098293 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6P67_RS16155 3098405..3098926 + 522 WP_001055874.1 flavodoxin FldB -
C6P67_RS16160 3098966..3099373 - 408 WP_000244772.1 protein YgfX Toxin
C6P67_RS16165 3099354..3099620 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6P67_RS16170 3099863..3100843 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6P67_RS16175 3100920..3101579 - 660 WP_000250274.1 hemolysin III family protein -
C6P67_RS16180 3101743..3102054 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6P67_RS16185 3102099..3103532 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
C6P67_RS16190 3103589..3104332 - 744 WP_001570285.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T98417 WP_000244772.1 NZ_CP027371:c3099373-3098966 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98417 NZ_CP027371:c3099373-3098966 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98417 WP_000354046.1 NZ_CP027371:c3099620-3099354 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98417 NZ_CP027371:c3099620-3099354 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References