Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2639045..2639670 Replicon chromosome
Accession NZ_CP027371
Organism Escherichia coli strain 2015C-3905

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag C6P67_RS13890 Protein ID WP_000911329.1
Coordinates 2639045..2639443 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag C6P67_RS13895 Protein ID WP_000450524.1
Coordinates 2639443..2639670 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P67_RS13870 2634952..2635152 + 201 WP_000383836.1 YpfN family protein -
C6P67_RS13875 2635233..2635931 - 699 WP_000679812.1 esterase -
C6P67_RS13880 2636005..2638020 - 2016 WP_106879148.1 tRNA cytosine(34) acetyltransferase TmcA -
C6P67_RS13885 2638035..2638898 - 864 WP_001267498.1 neutral zinc metallopeptidase -
C6P67_RS13890 2639045..2639443 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
C6P67_RS13895 2639443..2639670 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
C6P67_RS13900 2639825..2640538 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
C6P67_RS13905 2640751..2641785 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
C6P67_RS13910 2641802..2642680 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
C6P67_RS13915 2642826..2643398 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
C6P67_RS13920 2643398..2643868 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T98415 WP_000911329.1 NZ_CP027371:c2639443-2639045 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T98415 NZ_CP027371:c2639443-2639045 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT98415 WP_000450524.1 NZ_CP027371:c2639670-2639443 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT98415 NZ_CP027371:c2639670-2639443 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References