Detailed information of TA system TA098404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1209523..1209994
• Replicon: chromosome
• Accession: NZ_CP027371
• Organism: Escherichia coli strain 2015C-3905

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C6P67_RS06355
• Protein ID: WP_001303511.1
• Coordinates: 1209523..1209801 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C6P67_RS06360
• Protein ID: WP_001302048.1
• Coordinates: 1209803..1209994 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P67_RS06315	1204681..1204950	-	270	WP_000003742.1	excisionase	-
C6P67_RS06320	1205012..1207456	-	2445	WP_032284903.1	exonuclease	-
C6P67_RS06325	1207549..1207737	-	189	WP_000092784.1	DUF1482 family protein	-
C6P67_RS06330	1207734..1207922	-	189	WP_000450222.1	cell division inhibitor	-
C6P67_RS06345	1208451..1208966	+	516	WP_000559920.1	hypothetical protein	-
C6P67_RS06350	1209080..1209232	-	153	WP_000367379.1	DUF1391 family protein	-
C6P67_RS06355	1209523..1209801	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6P67_RS06360	1209803..1209994	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C6P67_RS06365	1210015..1210386	-	372	WP_001169685.1	hypothetical protein	-
C6P67_RS06370	1210483..1210785	+	303	WP_032279888.1	transcriptional regulator	-
C6P67_RS06375	1210782..1211207	+	426	WP_000693925.1	Rha family transcriptional regulator	-
C6P67_RS26495	1211276..1212307	+	1032	WP_044527394.1	phage replisome organiser	-
C6P67_RS06385	1212219..1212761	+	543	WP_072130322.1	replication protein	-
C6P67_RS06390	1212795..1213511	+	717	WP_106879105.1	DUF1627 domain-containing protein	-
C6P67_RS06395	1213544..1213825	+	282	WP_072094476.1	DNA-binding protein	-
C6P67_RS06400	1213822..1214127	+	306	WP_072094475.1	DUF4406 domain-containing protein	-
C6P67_RS06405	1214275..1214457	+	183	WP_001224672.1	hypothetical protein	-
C6P67_RS26670	1214450..1214626	+	177	WP_000753060.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2A / stx2B	1203594..1270415	66821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T98404 WP_001303511.1 NZ_CP027371:c1209801-1209523 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T98404 NZ_CP027371:c1209801-1209523 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCAGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTGGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT98404 WP_001302048.1 NZ_CP027371:c1209994-1209803 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT98404 NZ_CP027371:c1209994-1209803 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAGTTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0