Detailed information of TA system TA098399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4918444..4919069
• Replicon: chromosome
• Accession: NZ_CP027368
• Organism: Escherichia coli strain 2014C-3307

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6P71_RS25595
• Protein ID: WP_000911330.1
• Coordinates: 4918671..4919069 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6P71_RS25590
• Protein ID: WP_000450524.1
• Coordinates: 4918444..4918671 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P71_RS25565	4914248..4914718	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
C6P71_RS25570	4914718..4915290	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6P71_RS25575	4915436..4916314	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6P71_RS25580	4916331..4917365	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
C6P71_RS25585	4917578..4918291	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6P71_RS25590	4918444..4918671	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6P71_RS25595	4918671..4919069	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6P71_RS25600	4919216..4920079	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6P71_RS25605	4920094..4922109	+	2016	WP_000829355.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6P71_RS25610	4922183..4922881	+	699	WP_000679823.1	esterase	-
C6P71_RS25615	4922991..4923191	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98399 WP_000911330.1 NZ_CP027368:4918671-4919069 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98399 NZ_CP027368:4918671-4919069 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98399 WP_000450524.1 NZ_CP027368:4918444-4918671 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98399 NZ_CP027368:4918444-4918671 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33