Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4483189..4483843 Replicon chromosome
Accession NZ_CP027368
Organism Escherichia coli strain 2014C-3307

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C6P71_RS23430 Protein ID WP_000244781.1
Coordinates 4483436..4483843 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6P71_RS23425 Protein ID WP_000354046.1
Coordinates 4483189..4483455 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P71_RS23400 4478358..4479101 + 744 WP_000951961.1 SDR family oxidoreductase -
C6P71_RS23405 4479158..4480591 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
C6P71_RS23410 4480636..4480947 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6P71_RS23415 4481111..4481770 + 660 WP_000250274.1 hemolysin III family protein -
C6P71_RS23420 4481966..4482946 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
C6P71_RS23425 4483189..4483455 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6P71_RS23430 4483436..4483843 + 408 WP_000244781.1 protein YgfX Toxin
C6P71_RS23435 4483883..4484404 - 522 WP_001055874.1 flavodoxin FldB -
C6P71_RS23440 4484516..4485412 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6P71_RS23445 4485437..4486147 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6P71_RS23450 4486153..4487886 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T98397 WP_000244781.1 NZ_CP027368:4483436-4483843 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98397 NZ_CP027368:4483436-4483843 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98397 WP_000354046.1 NZ_CP027368:4483189-4483455 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98397 NZ_CP027368:4483189-4483455 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References