Detailed information of TA system TA098371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 65288..65913
• Replicon: plasmid unnamed
• Accession: NZ_CP027367
• Organism: Escherichia coli strain 89-3156

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6P65_RS26695
• Protein ID: WP_000911320.1
• Coordinates: 65515..65913 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2A2C6V4
• Locus tag: C6P65_RS26690
• Protein ID: WP_000450527.1
• Coordinates: 65288..65515 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P65_RS26685	64832..65206	-	375	WP_158249645.1	relaxase domain-containing protein	-
C6P65_RS26690	65288..65515	+	228	WP_000450527.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6P65_RS26695	65515..65913	+	399	WP_000911320.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6P65_RS26700	65922..68102	-	2181	WP_106884365.1	type IV conjugative transfer system coupling protein TraD	-
C6P65_RS26705	68384..68863	-	480	WP_001473214.1	hypothetical protein	-
C6P65_RS26710	69067..69798	-	732	WP_028985894.1	complement resistance protein TraT	-
C6P65_RS26715	69830..70327	-	498	WP_032211977.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..125561	125561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.21 Da
• Isoelectric Point: 7.8605

>T98371 WP_000911320.1 NZ_CP027367:65515-65913 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDATAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T98371 NZ_CP027367:65515-65913 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTACTGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTAACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8542.54 Da
• Isoelectric Point: 4.4013

>AT98371 WP_000450527.1 NZ_CP027367:65288-65515 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSDDFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT98371 NZ_CP027367:65288-65515 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGACGATTTTATGGATAACAGGGAACAGCCCGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A2C6V4